il2 elisa kit  (R&D Systems)

Journal: iScience

Article Title: Interferon-α and thymosin-α1 plus tislelizumab enhance CD8 + T cell cytotoxicity toward pancreatic ductal adenocarcinoma

doi: 10.1016/j.isci.2025.113053

Figure Lengend Snippet: Influence of tislelizumab, IFN-α, and Tα1 on the surface PD-1 expression of CD8 + T cells (A) Statistical analysis of the number of CD8 + T cells at different times within 14 days after incubation with 1 μg/mL of tislelizumab with or without IFN-α and Tα1 treatment, as determined by a CCK8 assay. (B–D) Statistical analysis of the levels of the secreted cytokines IL-2 (B), TNF-α (C), and IFN-γ (D) in suspensions of cultured CD8 + T cells after incubation with 1 μg/mL of tislelizumab with or without the addition of IFN-α and Tα1 for 14 days, as assayed by ELISA. The data were analyzed by one-way ANOVA and are presented as the means ± SDs of four or five biological replicates. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: IL-2 ELISA Kit , Sino Biological , Cat# KIT11848.

Techniques: Expressing, Incubation, CCK-8 Assay, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Sustained learned immunosuppression could not prevent local allergic ear swelling in a rat model of contact hypersensitivity

doi: 10.1101/2025.04.05.647348

Figure Lengend Snippet: ( A ) Schematic representation of conditioning paradigm and ( B ) group allocation. Animals underwent three acquisition trials. During these trials the CS, US, and CS 0 groups received saccharin and an injection with CsA (80 mg/kg) prior to sensitization with DNFB. After the incubation time, retrieval started with presentation of the saccharin solution ( CS and Veh group) or water ( US and CS0 group). The US group additionally received 80 mg/kg CsA. On the second day of retrieval, animals were challenged with DNFB and 24 h later ear thickness was measured. ( C ) Taste avoidance index. Conditioned animals showed a pronounced CTA on the second and third acquisition day and over the course of retrieval, reflected by a significantly lower saccharin consumption (ANOVA followed by Bonferroni post hoc analysis; ##p<0.01; ###p<0.001 = Veh vs. all groups; +++p<0.001 = all groups vs. acquisition day 1; ***p<0.001 = CS vs. all groups; n=6-12/group). ( D ) IL-2 levels of ex-vivo anti-CD3 stimulated splenocytes. Compared to the CS0 group, US and CS animals showed reduced IL-2 amounts. Data are presented as percentage of CS0 group (ANOVA followed by Bonferroni post hoc analysis; *p<0.05, ***p<0.001; n=6-12/group). Data are shown as mean ± SEM.

Article Snippet: IL-2 amounts of the supernatants were measured using a sandwich ELISA (Quantikine®ELISA Rat IL2, R&D systems, Minneapolis, USA) according to the manufacturer’s instructions.

Techniques: Injection, Incubation, Ex Vivo

Journal: bioRxiv

Article Title: Sustained learned immunosuppression could not prevent local allergic ear swelling in a rat model of contact hypersensitivity

doi: 10.1101/2025.04.05.647348

Figure Lengend Snippet: ( A ) Schematic representation of conditioning paradigm and ( B ) group allocation. During three acquisition trials (CS/UCS parings), animals of all groups ( CSlow , USlow , US , CS0 ) received saccharin and an injection with CsA (80 mg/kg). Subsequently, animals were sensitized with DNFB. 5 days following sensitization, conditioned animals ( CSlow ) received saccharin paired with injections of 25 % (20 mg/kg) of the full therapeutic CsA dose during retrieval. Control groups received either water together with injections of either 20 mg/kg CsA ( USlow group) or 80 mg/kg CsA ( US group), or vehicle ( CS0 group). Animals were challenged with DNFB on the second day of retrieval. ( C ) Taste avoidance index. Compared to all other groups, conditioned animals ( CSlow ) displayed a pronounced CTA, over the course of retrieval (ANOVA followed by Bonferroni post hoc analysis; +p<0.05, +++p<0.001 = all groups vs. acquisition day 1; ***p<0.001 = CSlow vs. all groups; n=5-6/group). ( D ) US and CSlow groups also significantly differed in IL-2 production of ex-vivo anti-CD3 stimulated splenocytes compared to CS0. Data are presented as percentage of CS0 group. (ANOVA followed by Dunnett’s post hoc analysis, +p<0.05; ++p<0.001; *p<0.05, ***p<0.001; n=5-6/group; Data are shown as mean ± SEM).

Article Snippet: IL-2 amounts of the supernatants were measured using a sandwich ELISA (Quantikine®ELISA Rat IL2, R&D systems, Minneapolis, USA) according to the manufacturer’s instructions.

Techniques: Injection, Control, Ex Vivo